1 1561-4107 Journal of Babol University Of Medical Sciences 4041 Biochemical The Interactive Effect of Lead Acetate and Endurance Training on the Brain-Derived Neurotrophic Factor and Malondialdehyde Levels in Rats Cortex Hosseinzadeh S Dabidi Roshan V Mahjoub S Taghipour Darzi M 1 3 2012 14 2 7 15 08 06 2014 BACKGROUND AND OBJECTIVE: Studies show that the moderate intensity exercise reduces the oxidative stress and increases the neurotrophin levels in brain, but less attention was given to the examination of exercise-induced neuroprotection after administration of lead acetate. The purpose of this study was to investigate the effects of 8 week endurance training on the brain-derived neurotrophic factor (BDNF) and malondialdehyde (MDA) levels in rats cortex exposed to lead acetate.METHODS: In this experimental study, 40 male Wistar rats, 50 days old with average weight of 250±3.79 g, were randomly divided into four groups included the base, control, exercise+lead and lead groups. The exercise training protocol consisted of running on a treadmill for 8 weeks training (15-22 m/min, 25-64 min). Exercise-lead and lead groups received 20 mg/kg lead acetate and sham group received 30 mg/kg of ethyl oleate peritoneally for 8 weeks. BDNF and MDA levels in cortex were measured by ELISA and TBARS methods, respectively. FINDINGS: Although, induction of lead acetate didn’t lead to significant change in cortical BDNF levels in lead group in compared to the control (1.93±1.26 in lead group in comparison to 1.78±1.13 ng/mg protein in control) but caused a significant increase in MDA levels in cortex in compared with control group (0.61±0.07 in lead group in comparison to 0.31±0.05 nmol/mg protein in control) (p values for BDNF and MDA: p=0.994 and p=0.000, respectively), versus the 8-week training reversed this process so that, caused insignificant increase in BDNF (2.98±1.39 in lead group in comparison to 1.93±1.26 ng/mg protein in control) and significant reduction in cortical MDA in training group in compared to lead group (0.5±0.04 in lead group in comparison to 0.61±0.07 nmol/mg protein in control) (p values for BDNF and MDA: p=0.207, p=0.048, respectively).CONCLUSION: The results of this study showed that although, regular exercise didn’t increase the BDNF significantly, but it can strengthen the brain’s defense structure against air pollutants through preventing BDNF reduction and improving oxidant/antioxidant process.
4042 Biochemical Study of Environment Temperature Effect on the Antibacterial Activity of Water Extract of Different Organs of Viola Odorata in the Different Stages of Growth Ramezani M Zarrinkamar F Bagheri M Rajabnia R 1 3 2012 14 2 16 21 08 06 2014 BACKGROUND AND OBJECTIVE: Viola odorata is an important medicinal plant that used for the treatment of bronchitis, common cold and digestive diseases. Nowadays, due to the spread of bacterial resistance to antibiotics more attention is given to the activity of antimicrobial medicinal plants. The aim of this study was to assess the effect of environment temperature on the antibacterial activity of aqueous extracts of different organs at different stages of viola odorata growth.METHODS: In this experimental-laboratory study, plant after growth in three temperatures (10, 20 and 30 degree) was collected. Extraction was performed by percolation method, and antibacterial activity of extracts was investigated on three bacteria (Staphylococcus aureus, E. coli, Pseudomonas aeruginosa with disk-diffusion and macrodilution methods. FINDINGS: Aqueous extract has antibacterial activity on all three bacteria so that has maximum effect on Staphylococcus aureus (concentration of 1µgr/ml) and minimum effect on Pseudomonas aeruginosa (concentration of 8µgr/ml). Evaluation of antibacterial activity of organs showed that cold treatment has more effect than warm and control treatment. With plant growth, antibacterial activity decreases in leaf and root, but in flowering stage it increases in flower organ.CONCLUSION: Results of study showed that water extract of the plant organs in two different temperatures on three bacteria has effective antibacterial activity. So, more effective components can be collected by creating optimal conditions for growth and using specious organs. 4043 Biochemical Interactive Effect of Endurance Exercise and Crude Alcoholic Extract of Magnolia on Liver Interleukin-6, Interleukin-10, Glucose, and Glycogen in Male Rats Farzanegi P Abdi M Ghanbari-Niaki A Fathi R Hedayati M 1 3 2012 14 2 22 30 08 06 2014 BACKGROUND AND OBJECTIVE: Use of effective supplements on physical function in comparison to industrial supplements is important for a lot of sports nutrition scientists. The purpose of this study was to investigate the interactive effect of endurance exercise and crude alcoholic extract of magnolia on interleukin-6, interleukin-10, glucose, and glycogen in male rat liver.METHODS: In this experimental study, twenty one 6-8 week male rats were divided into 3 groups of saline-control, saline training and magnolia training. The training groups ran on treadmill for 8 weeks (60 min/d, 5d/wk at 25 m/min and 0% grade). Magnolia extract and saline in equal volume (2 mg per kg body weight) from the beginning of the second week for 4 weeks (5 days per week) were fed to three groups. Effect of extract on Il-6 and Il-10 were measured with ELISA method, and glucose and glycogen were measured with Colorimetric method. FINDINGS: IL-10 in practice groups (8.23±1.57 pg/ml versus 25.04±3.05 pg/ml) were significantly lower (p=0.0001). While, Il-6 in saline training group (208.43±48 pg/ml) showed a significant increase in compared to control group (151.29± 23.08 pg/ml) (p=0.04). Glucose level (75.14±24.64 mg/dl versus 87.29±15.07 mg/dl) and glycogen level (3.74± 0.6 mg/g versus 4.67±0.51 mg/g liver tissue) in saline training group were significantly lower (p=0.114 and p=0.024, respectively).CONCLUSION: The results of this study showed that the improvement of liver glycogen induced by magnolia could prevent from exercise-induced increase in IL-6. 4044 Biochemical Association of p53 Arg/Pro Polymorphism at Codon 72 with Risk of Breast Cancer in East Azerbaijani Women Hossein Pour Feizi MA Ravanbakhsh Gavgani R Pourahmad R Pouladi N Azarfam P Montazeri V 1 3 2012 14 2 31 38 08 06 2014 BACKGROUND AND OBJECTIVE: Breast cancer is most common cancer among women in the world and one third women affected by this cancer. P53 gene is one of the most important tumor suppressor genes. Investigations show G to C polymorphism at codon 72 of p53 gene, is associated with the increasing of cancer, and perhaps it can be regarded as a predisposing marker for breast cancer. This study was done to assess the association of p53 Arg/Pro polymorphism at codon 72 with risk of breast cancer in women.METHODS: This case-control study was conducted on 126 patients with invasive and insitu, as well as 99 control individuals as controls from Tabriz hospitals, DNA extraction was conducted using proteinase K method, different genotype of codon 72 of gene p53 were determined using allele-specific polymerase chain reaction (PCR) and direct DNA sequencing and compared. FINDINGS: In the control group, the genotype distribution of p53 polymorphism, showed 30.3%, 50.5% and 19.2% for the Arg/Arg, Arg/Pro, Pro/Pro Genotypes, respectively. In the cancer group, the distribution was 44.4%, 34.9% and 20.6% for the Arg/Arg, Arg/Pro and Pro/Pro genotypes, respectively. Distribution differences in the p53 codon 72 polymorphism between the cases and controls were statistically significant (p<0.05).CONCLUSION: This study indicates that p53 codon polymorphism is a genetic predisposing factor for breast cancer in East Azerbijan’s women. However, further studies are needed to determine the role of p53 codon 72 polymorphism in breast cancer. 4045 Biochemical Evaluation of Vasorlaxant Effect of Achillea Wilhelmsii Hydroalcholic Extract on Isolated Aorta in Rat Harandizadeh F Hosseini SM Behnam Rasouli M Niazmand S 1 3 2012 14 2 39 46 08 06 2014 BACKGROUND AND OBJECTIVE: Achillea is well known herb in traditional medicine which has anticonvulsant and tonic effects, however it is used to treat tachycardia and angina pectoris. Some reports of antihypertensive and hypotensive effects of Achillea wilhelmsii are published. This study aimed to investigate the vasorelaxant effect of Achillea wilhelmsii hydroalcholic extract on isolated aorta in rat.METHODS: It was an interventional study in which 42 male Wistar rats (weighed 200-250 g) were divided randomly into 6 groups. In groups 1 and 2 the effect of the extract (1, 5 and 25 mg/ml) on contracted aorta by phenylephrine in intact and denuded endothelium were investigated. In groups 3 and 4 the effect of the extract on contracted aorta by chloride potassium in intact and denuded endothelium were investigated. In groups 5 and 6 the effect of the extract (5 mg/ml) on contracted aorta by phenylephrine and chloride potassium in the presence of cumulative calcium concentrations were evaluated by isolated tissue setup. FINDINGS: In intact aorta contracted by phenylephrine, the extract relaxed 8%, 47% (p<0.001) and 45% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In denuded endothelium aorta contracted by phenylephrine the extract relaxed 16% (p<0.05), 56% (p<0.001) and 54% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In intact aorta contracted by chloride potassium the extract relaxed 18% (p<0.01), 57% (p<0.001) and 57% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. In denuded endothelium aorta contracted by chloride potassium the extract relaxed 2.5%, 47% (p<0.001) and 54.5% (p<0.001), respectively, at concentrations 1, 5 and 25 mg/ml. The extract (5 mg/ml) relaxed 7% (p<0.001), 12% (p<0.001), 22% (p<0.001) and 31.5% (p<0.001). The extract (5 mg/ml) relaxed 5.5% (p<0.01), 9.3% (p<0.001), 19% (p<0.001) and 74% (p<0.001) the contracted aorta by chloride potassium in the presence of cumulative calcium concentrations (from 10-5 to 10-2 respectively).CONCLUSION: The extract showed endothelium-independent vasorelaxant effect. The relaxation mainly was mediated by inhibition of voltage- and receptor-dependent Ca2+ channels in vascular smooth muscle cells. 4036 Biochemical Correlation between Frequency of P53 and P21 Proteins with Pathological Parameters in Colorectal Adenocarcinoma Jahantigh M Narouie B Sheikhi Ghayur E Davarian A 1 3 2012 14 2 47 52 08 06 2014 BACKGROUND AND OBJECTIVE: Colorectal cancer is the major and cause of morbidity and mortality throughout the world. Loss of activity of P53 and P21 (WAF1) proteins that belong to the cell cycle regulation family of protein, seems to be important regulatory mechanism of carcinogenesis in colorectal cancer and affected prognosis and resistance to chemotherapy. P21 expressed in P53-dependent and independent pathway. This study was designed to assess P53 and P21 frequency and their correlation with pathological parameters in colorectal adenocarcinoma.METHODS: This analytical study was done on 70 paraffin preserved colorectal adenocarcinoma samples of patients in Zahedan, Iran who underwent colectomy from 2003 to 2010. Tissue slice was stained and evaluated by method of immunohistochemistry for P53 and P21 protein and evaluated. FINDINGS: P53 was expressed in 37( 52.9%) patients and P21 was expressed in 33(47.1%). No correlation was found between P53 overexpression and P21 with pathological variables. P21 had a statistically significant association with tumor differentiation (p=0.02). The high frequency of P21 was seen in good differentiated tumors.CONCLUSION: The results of the study show that P53 mutation plays an important role in the development of colorectal cancer. No correlation between P21 expression and P53 statue supports the theory that P21 induction occurs mostly in P53 independent pathway in colorectal cancer. 4046 Biochemical Renal Scaring in Infants with Prenatal Hydronephrosis and Vesicoureteral Reflux Sorkhi H Mojtabaei M 1 3 2012 14 2 53 58 08 06 2014 BACKGROUND AND OBJECTIVE: Primary vesicoureteral reflux (VUR) is a hereditary disease and the risk of scar increased with VUR. In a few years ago, probability of renal scar formation before delivery and urinary tract infection is suggested. So, this study was done to evaluate this theory and detection of renal scar in neonates with prenatal hydronephrosis and VUR.METHODS: This cross sectional study was performed on 59 neonates with history of prenatal hydronephrosis. The hydronephrosis were proved after delivery and VCUG (voiding cystourethrography) was done with negative urine cultures. For detection of scar (decrease radioisotope absorption), DMSA (Dimercaptosuccinic Acid) was done in all neonates with VUR. Scar in grade I-V was assessed.FINDINGS: This study was done in 59 neonates with history of prenatal hydronephrosis and VUR. Thirty nine (66.1%) neonates were boy and others were girl. Among 116 kidney units, 95 units had VUR and 51 (53.7%) units had scar. Thirsty nine (62%) units with VUR in boys and 12 (37.5%) units in girls had scar (p<0.05). Also, none of units with grade I had scar. Scar in grade II-V was 37.5%, 42.1%, 48.1% and 88.5%, respectively (p<0.05).CONCLUSION: The existence of high rate scar in these neonates, especially in males and with high grade VUR indicated that early diagnosis and also prevention of UTI and new scare formation is very important. 4037 Biochemical Comparison Study of Behavioral Activation System and Behavioral Inhibition System in Irritable Bowel Syndrome Patients and Normal Individuals Kalantari H Bagherian-Sararoudi R Babaeipour E 1 3 2012 14 2 59 65 08 06 2014 BACKGROUND AND OBJECTIVE: The evidence confirms the link between personality traits and psychosomatic diseases including irrational bowl syndrome. In recent years, a psychophysiological approach of personality has focused on behavioral activation system and behavioral inhibition system. The purpose of this study was to compare these behavioral systems between normal people and the patients with Irrational Bowl Syndrome.METHODS: In a case-control study, 80 consecutive patients with established IBS referred to two gastroenterology clinics in city of Esfahan, Iran and 80 healthy people matched regarding demographic variables were selected. They filled out brain/behavioral questionnaires of Carver and White. Then two groups were compared regarding brain/behavioral systems activities.FINDINGS: The results of ANCOVA showed that the scores of behavioral inhibition system in patients (22.34±0.329) were significantly higher than normal people (20.98±0.329) (F=8.419, p=0.004). However, there was no significant difference between two groups in behavioral activation scores (F=0.234, p=0.63).CONCLUSION: The finding support increased activity of inhibition system in patients with irritable bowel disease. It means increased sensitivity of IBS patients to punishment and threatening events. This condition may cause decreased pleasure experience and increased negative affects in IBS patients. 4047 Biochemical Skin Prick Test with Aeroallergens in Patients with Chronic Urticaria Ghaffari J Mohammadzadeh E Mahdavi MR 1 3 2012 14 2 66 72 08 06 2014 BACKGROUND AND OBJECTIVE: Aeroallergens induce exacerbation of chronic urticaria. Skin prick test is a suitable test for detection of immediate reaction. The aim of this study was to detect the incidence of positive skin prick test to aeroallergens in patients with chronic urticaria.METHODS: This cross-sectional study was done on patients with chronic urticaria during three years. After taking history and physical examination of patients, laboratory tests including: CBC-diff, ESR, stool exam, thyroid test, CH50, C3, C4, IgE, ANA, simple urinary test, AST and ALT were done. We used aeroallergens including: Timothy, Bermuda, Maple, Nettle, Feather, Aspergillus, Alternaria, Cockroach, Grass mixed, Ash, Alder, Russian Thistle, Pigweed, Dermatophagoides Farinae, Dermatophagoides pteronyssinus with negative and positive tests on the forearm. The result was evaluated.FINDINGS: From 78 cases with chronic urticaria, 23 (30%) were male and 55 (70%) were female. Their age was between 7 to 50 years old and the mean age was 30.43±11.27 years. Twelve (15%) patients had positive skin prick test to 9 allergens that 8 (10%) were male and 4 (5%) were female. Among positive skin prick tests, 9 of 25 cases were related to Dermatophagoides pteronyssinus (DP) (36%) and 9 cases were related to Dermatophagoides farinae (DF) (36%). CONCLUSION: The results showed that mites are more common in chronic urticaria. 4038 Biochemical Relationship between Negative Affectivity and Severity of Irritable Bowel Syndrome (IBS) Symptoms Bagherian-Sararoudi R Kalantari H , Afshar H Daghaghzadeh H Abotalebian F Falah J Adibi P 1 3 2012 14 2 73 81 08 06 2014 BACKGROUND AND OBJECTIVE: Irritable bowel syndrome (IBS) is one of the common gastrointestinal disorders considered as a psychosomatic disorder. Type D personality characterized by both global traits including negative affectivity and social inhibition has been proposed to be related to etiology of psychosomatic disorders. However, the role of this personality type in severity of IBS symptoms remains unclear. The purpose of this study was to examine the role of type D personality in severity of IBS symptoms.METHODS: In a cross sectional study 194 consecutive patients with IBS referred to the psychosomatic clinics at Noor hospital, Isfahan were selected from Winter 2009 to Summer 2009. The patients were divided into two groups with (79 patients) and without (115 patients) type D personality by using DS-14. The patients completed type D personality Scale (DS-14) and the IB Severity Scoring System (IBS-SSS). Then the data was analyzed.FINDINGS: The findings indicated that 40.7% were the type D personality. Although the results of ANCOVA showed no significant difference between two groups, with and without type D, in severity of IBS symptoms (F=1.39, p= 0.24), multiple stepwise regression analysis indicated negative affectivity, as one of the type D dimensions (β= 4.03, p= 0.006) and treatment duration (β= 0.428, p= 0.038) were the significant predictors for severity of IBS symptoms.CONCLUSION: Although, it seems that type D personality as a psychological factor does not effect on IBS symptom severity, the evidence confirms possible role of negative affectivity as one of the type D dimensions in severity of IBS symptoms. 4039 Biochemical Outcome of 200 Kidney Transplantation in Kidney Transplant Center of Shahid Beheshti Hospital in Babol (Iran) Akbarzadeh Pasha A Sorkhi H Razzaghi E Oliaei F Gholizadeh Pasha A Alizadeh-Navaei R Gheibi B 1 3 2012 14 2 82 88 08 06 2014 BACKGROUND AND OBJECTIVE: Kidney transplantation is a choice treatment for most patients with end-stage renal disease and transplantation outcome is different in kidney transplant centers. This study was done to analyze the results of kidney transplantation in kidney transplant center of Shahid Beheshti hospital in Babol, Iran.METHODS: This survival analysis study was performed on the results of the first 200 renal transplantations during 5 consecutive years. Data including age, sex, 1, 2 and 3 years graft and patient survival, kidney transplant recipients, nephrologic and urologic complications, and creatinine distribution at end of 1, 2 and 3 years after transplantation were gathered and evaluated.FINDINGS: The mean age of patients was 39.34±15.1 years, and 116 (58%) of patients were male. 1, 2 and 3 year graft survival was 98.86%, 97.3% and 97.3%, respectively and 1, 2 and 3 year patient survival was 96.63%, 96.63% and 93.12%, respectively. Treatment of clinical acute rejection without pathological diagnosis was performed on 19 patients (9.5%) and 16 patients responded to treatment. Acute tubular necrosis and gradual decrease in creatinine was seen in 13%. The surgical complication was seen in 3.5%.of patients who respond to treatment. At the end of first year, 0.5% of patients had creatinine more than 2.CONCLUSION: The results of this study showed that the short term graft survival in kidney transplant recipients was considerable. 4040 Biochemical Testicular Metastasis from Prostate Carcinoma: A Case Report Shafi H Rafati Rahimzadeh M Pourdadash B Firouzjahi AR 1 3 2012 14 2 89 94 08 06 2014 BACKGROUND AND OBJECTIVE: The testis is a rare site for prostate cancer metastases and is usually found in autopsies or incidentally after therapeutic orchiectomies. The prognosis for such patients is very poor. Bilateral orchiectomies were done for patients. The aim of this report was to introduce one case of testicular metastases from prostate carcinoma.CASE: The patient was a 75 year old male that has prostatism signs. Primary examination showed the increase of prostate-specific antigen (PSA). Biopsy and nuclear scan were requested for patient. Pathology reported multifocal infiltrating prostate adenocarcinoma, also estimated Gleason score of 4+5=9 and vertebral column involvement. The patient was candidate for bilateral orchiectomies and ten sessions of radiation for vertebral column was requested. After pathology feature and immunohistochemical (IHC) study for testis, deposits of carcinoma were posed. Now, symptoms related to bone pain are decreased, and PSA=68 ng/ml change to PSA=6 ng/ml. CONCLUSION: Considering the reported case, evaluation in patients with prostate carcinoma by clinical examination, pathology assessment of testis tissue and biomarkers seems to be necessary.