1 1561-4107 Journal of Babol University Of Medical Sciences 2906 Biochemical Protective effects of Selenium on Lead impacts in functioning of submandibular salivary gland of rats Abdollahi M Rahmat Girdeh N Marzban M 1 4 2000 2 2 8 15 25 05 2014 Objective: Animal experiments have demonstrated that selenium can partly reduce nephrotoxicity and neurotoxicity of lead. There is no study about possible interactions between lead and selenium in functioning of salivary glands. This study was designed to explore the possible protective effects of selenium on lead-induced alterations on rat submandibular gland function. Methods: Pure submandibular saliva was collected intraorally by micro polyethylene cannula from anaesthetized rats using pilocarpine as secretagogue. Findings: A twenty-four day treatment with lead 0.04% and 0.05% as acetate in drinking water altered salivary function. Flow rate was significantly increased by lead (0.04% and 0.05%, P<0.01). The total protein and calcium concentrations of saliva were decreased significantly by either doses of lead (P<0.01). Also both doses of lead decreased the activity of NAG significantly (P<0.01). Selenium alone treatment (2.5 mg/L) in drinking water for 24 days did not induce any significant change in saliva secretory function but treatment with lead and selenium compensated the lead-induced decrease in NAG activity and concentrations of calcium and protein significantly at P<0.01 and raised them to levels close to those in controls. Increased flow rate by lead acetate was also affected by selenium co-treatment and reached that level in control. Conclusion: It is concluded that selenium can prevent lead-induced adverse effects on rat submandibular gland function. Properties of selenium as antioxidative, free radical scavenger and maintenance of cell membrane integrity are possible mechanisms of its protective effects. Further studies about interaction of lead and selenium in salivary glands at molecular level are proposed to explore the exact mechanism of their interaction.
2907 Biochemical The study of correlations between B-Lactamase and protein A in Staphylococcus aureus Kasra Kermanshahi R Keyvani Amineh H Gol Alipour M 1 4 2000 2 2 16 22 25 05 2014 Objective: Staphylococcus aureus is the type species and pathogen of human. There is some factors that determines the virulence of bacteria, but protein A and B-Lactamase are the most important factors. We studied the correlations between two factors of virulence (B-Lactamase and protein A) in this microorganism. Methods: We studied the resistance to B-Lactamase sensible to B-Lactamase as penicillin G (Pen G), Cefazolin (CeZ), Cefalotine (Cfn), resistant to B-Lactamase as methicillin (Met) and Oxacilin (Oxa) with disk diffusion method. MICS and B-Lactamase production detected by broth dilution and acidometric methods. Purification of SPA performed on these samples with ion exchange chromatography method (DEAE-Cellulose). Cell wall lysis was performed with heat extraction method. Findings: These results showed that resistance to drugs was 96% (Pen G), 83% (CeZ), 86% (Cfn) and 73% (Oxa). Purification performed on these samples with method (DEAE-Cellulose) have a sharp dense band in electrophoresis (SDS-PAGE) with a molecular weight of about 42 kd. Conclusion: After studying relationship between existence of SPA and B-Lactamase, we determined that 93.7% of strains with B-Lactamase had SPA but the strains without B-Lactamase only 50% had SPA. The result with methicillin resistant strains is most representative because in these kinds of strains there are not any strains with SPA. 2908 Biochemical Pain threshold changes in 10-70 year old healthy female in Shiraz Ghafari Nezhad F 1 4 2000 2 2 23 26 25 05 2014 Objective: Pain is a protective mechanism for the body that occurs after tissue damage. This mechanism is influenced by many factors. This study is performed to detect of pain threshold and the effect of aging on it. Methods: This study was conducted on 360 normal females between 10-70 years old that were divided to six decades. The pain threshold was measured from the Ching ling’s acupuncture point on medical of arm by use of electrical stimulator (250ms, 5HZ). Statistical analysis was done by ANOVA method and T student. Findings: The results showed that the highest average of pain threshold observed at age group of 51-60 year old and it’s least rate observed at age group of 21-30 year old. Conclusion: There is no significant correlation between pain threshold, and aging process. 2909 Biochemical A comparison of hemodynamic effects of Midazolam and Thiopental in induction of anesthesia Alijan Poor E Rabiei SM Mir Shekari S 1 4 2000 2 2 27 32 25 05 2014 Objective: Although thiopental sodium (Nesdonal) is an almost ideal drug for induction of anesthesia, but it mostly cause hemodynamic changes such as hypotension. The objective of this study is to compare hemodynamic effects of thiopental sodium and Midazolam (From Benzodiazpine family) in order to replace it with thiopental when necessary. Methods: 50 patients in ASA I were divided into two groups (Each group 25 persons) randomly subjects in both groups were administered with premedication with an equal dose per patient’s weight. In first group anesthesia was started with thiopental sodium 4-5 mg/kg with succinylcholine and in the second group with Midazolam 0.25-0.3 mg/kg with succinylcholine and intubation was done after a given time. Heart rate and blood pressure were recorded for both groups at special times and data were compared with each other. Findings: In this study pulse rate of 20% of patients who received thiopental sodium were increased to 20 beats/min. It was 12% for Midazolam group the changes (P=0.000) were meaningful. An increase of systolic blood pressure more than 40 mmHg from base after intubation with thiopental sodium in 24% of persons (P=0.011) and 2 min after intubation in 20% persons (P=0.048) were meaningful. There were no such variations in Midazolam group. Data shows that any rise or fall in blood pressure and heart rate observed with thiopental sodium is more than that with Midazolam. Conclusion: Considering this research and the other studies show more record of hemodynamic condition of Midazolam than thiopental sodium. Therefore, in patients with dangerous hemodynamic variations, especially decreasing of blood pressure only induction with Midazolam or a combination of Midazolam and thiopental (For rapid onset action) as co-induction is useful. 2910 Biochemical A comparative survey of ligation of uterine tubes by laparoscopy Filshie Clips and Modified Minilaparotomy Pomeroy technique Seidi Alavi Gh Saghafi N Pakdaman M 1 4 2000 2 2 33 39 25 05 2014 Objective: To compare two methods of sterilization, 196 women who intended to undergo tubal sterilization, were randomly assigned to sterilization either by Filshie clips laparoscopy (96 cases) or modified Pomeroy minilaparotomy technique (100 cases). Methods: We compared the time of operation, surgical complication, post operative pain and wound infection and after a long term follow up of (1-4 years) compared the changes in menstrual indices, pelvic pain and method failure. Findings: With the exception of severe and moderate postoperative pain, no statistically significant difference could be identified after evaluation of other complications (P<0.05). The failure rate was 1.72% for clips laparoscopy group but no pregnancy was found in pomeroy minilaparotomy group. Minilaparotomy however, needs less equipment and training. Regarding safety and effectiveness of the method and fewer post-surgical complications. However, the differences found in this study are not so significant. If can individual physician prefers this surgical approach, then the results will be favorable. Conclusion: This method seems to be considerably advantageous. 2911 Biochemical Improving the cerebrospinal fluid test (LP) in Amirkala Pediatric Hospital, 2000 ZahedPasha Y 1 4 2000 2 2 40 45 25 05 2014 Objective: Neonatal meningitis is more common than the other ages and it’s associated with neural complications and high mortality rate. The cerebrospinal fluid test is the most important test in the diagnosis of meningitis. According to several reports, there were some problems in reporting of this test in Amirkola pediatric hospital. Using the CQI model for quality improvement of cerebrospinal fluid reports to decrease processes time taking, complications and increase the satisfaction. Methods: This is a quasi-experimental (Pre and post) study performed by FOCUS-PDCA in Feb-2000 in Amirkola pediatric hospital. The time taking of 25 CSF samples reports were measured randomly, then the improvement program performed and the time were measured again in 25 other CSF samples reports and the results were evaluated. Findings: Not having a regular program to remit the samples was the most important cause of low quality according to numerous vote. The pre-improvement median time took 3:30 hours and the post one took only 40 minutes. Conclusion: According to our results the process time taking decreased 2 hours and 50 minutes after improving program. It caused low complications and high satisfaction. We believe that this first effort can provide a model for the expansion of CQI not only in Babol university of medical sciences but throughout Iran. 2912 Biochemical The prevalence of Leprosy in Kermanshah province in 1981-98 Tahriri Sh 1 4 2000 2 2 46 49 25 05 2014 Objective: Leprosy is endemic in many parts of the world including Iran. To data there has been no study on the prevalence of leprosy in Kermanshah province. Methods: A descriptive study was performed on the clinical history of 218 leprosy patients referring to the Kermanshah leprosy center from 1981-1998. Findings: 74.4% of the patients are male and 26.6% female. The average age of males is 42 years old and females 38. Male to female ratio is 3/1. The highest incidence of leprosy has shown to be among 50-59 years old patients. Kermanshah city and Javanrood have the highest and Sarpolzahab the lowest rate. Lepromatous leprosy (LL) and then the borderline leprosy (BL) are the most common clinical types. Conclusion: Leprosy is endemic in Kermanshah province. The center of the province and Javanrood have the highest rate. Therefore it is necessary to consideration that in Kermanshah province. 2913 Biochemical Assessment of infertility reasons & some effective factors on infertile couple, Babol, 1999 Bakhtiari A 1 4 2000 2 2 50 56 25 05 2014 Objective: One of the significant problems in modern gynecology is infertility that may cause emotional stresses leading to destructive effects on community and family. In order to find a solution to this social problem and to evaluate the causes of infertility we conducted this study. Methods: This is a cross-sectional (Descriptive-analytical) study using individual medical history, clinical examination and paraclinical investigation. In this evaluation 920 files of couples referred to Babol infertility center were used. At the first time variables such age couples, type of infertility, duration of infertility, family relation of the couples, family history, and family causes infertility of with respect to sex were investigated. Correlation of these variables and with causes of infertility was investigated. Findings: 76.8% have primary infertility. 78.5% of women and 80% of men were between 20-34 and 25-36 years old, respectively. More than 30% of couples had a family history of infertility specially in their immediate family. The average of infertility duration was 5.6±4.25 years. On the whole, 43.3% of the causes of infertility were associated with the females, and for men it was 22.6% and 27.2% for both sexes, 7% were diagnosed to have idiopathic infertility. The most significant factors of infertility were found to be, ovulation disorders (45.7%) and uterine cause (4.6%) among women and idiopathic asthenospermia and azoospermia due to insufficient primary testis (8.8%) for men. This investigation shows a meaningful relation between age and duration of infertility of any kind. Despite the meaningful relation between causes of infertility and factors such as age, duration and the kind of infertility in women, age was not a significant factor in men. Conclusion: With respect to the high prevalence of primary infertility and ovulation disorder and azoospermia, compared to other studies, treatment strategies should be planned to defect and manage the causes. However, factors such as age, duration and kind of infertility should relating to infertility should necessarily be considered in its medical intervention. 2914 Biochemical A case report of A-Beta Lipoproteinemia Farahmand F 1 4 2000 2 2 57 59 25 05 2014 Objective: A-beta lipoproteinemia is a genetic disease with recessive autosomal inheritance beginning with digestive symptoms like diarrhea and malabsorption and progressing with growth impairments in patient’s appearance. Early diagnosis and treatment remarkably reduces neurologic and eye disorders. Case: A 7-month baby was admitted to digestive disease unit of children medical center for his growth impairment. During infancy he had digestive symptoms and after 5 years of age developed neurologic and ophthalmic symptoms (Disorders) with reduced triglycerides and cholesterol levels in serum. Severe acanthocytosis was detected in his peripheral blood smears. Conclusion: Diagnosis was based on clinical manifestations, special histopathologic changes of duodenum and acanthocytosis of peripheral blood smear. Early diagnosis and proper treatment of A-beta lipoproteinemia relieves gastrointestinal disorders and neurologic symptoms. Meanwhile it prevents early death of the patients.