1 1561-4107 Journal of Babol University Of Medical Sciences 2662 Biochemical Effect of Phosphorous on the rate of Acid Phosphatase in taste bud cells in papilla of rat tongue Radfar A Hamzeh A Hajian K Pour Amir M 1 10 2004 6 4 7 11 31 05 2014 Background and Objective: There are 3 sensitive cells with a few basal cells in the rat tongue (Dark cell I, light cells II and III). The aim of this study was to determine the effect of different concentrations of phosphate on the rate of Acid Phosphatase in taste bud cells in papilla of rat tongue. Methods: To determine the influence of Phosphorous on Acid Phosphatase in taste buds, the dose of 0.5, 1.5, 3 and 6% and control group of NaH2PO4, 2H2O in the drinking water were used for 30 days then all rats were killed, and their tongues were isolated for histological examination. Black granules in the papillary cells (Cytoplasm) of rat taste buds due to plumbsulfhydril from the Phosphorous and Acid Phosphatase with staining region were counted. Gomori’s staining method and counting black granules were statistically analyzed with non-parametrical of Kruskal-Wallis and ANOVA. Findings: Based on salts were used in the water There was a linear relationship between the concentration of salt and the rate of Acid Phosphatase. It was seen a significant difference in mean of black granules in taste bud cells in 5 different groups of food Phosphorous (P<0.0001). Conclusion: There is a direct relationship between the concentrations of salt in the drinking water with the granules in the taste bud cells.
2663 Biochemical Preparation and standardization of pharmaceutical beeswax Asgari Rad H Pour Morad F Akbari KH 1 10 2004 6 4 12 16 31 05 2014 Background and Objective: Due to a very specific and unique characteristics of beeswax, it is important and suitable to use in pharmaceutical and cosmetic industries as a coating and slower agent in dissolution of pharmaceutical compounds. Regarding to the important role of beeswax, it has been decided to evaluate the preparation of the pharmaceutical beeswax in laboratory. Methods: The raw beeswax was provided from the waxes prepared by Appis mellifera in 2 different seasons (Fall and spring). Wax was melted by several methods using hot water, ether and ethanol 90%, in a controlled temperature. The same procedure was performed to prepare the white wax in presence of a bleaching agent. The wax sheets were analyzed and standardized under British pharmacopoeia (BP) protocols. Findings: Beeswax extraction with different solvents showed that using ether for preparing spring and fall yellow wax, some indices were out of standard limits by regarding to white wax that was prepared by hot water we could get a significant difference. Using ethanol as a solvent for extraction is not suitable and decreases the indices significantly. Conclusion: The spring white and yellow wax which were prepared by hot water method in a temperature lower than 85°C is recommended as a suitable and economical method with the same results comparing with BP standard beeswax. 2664 Biochemical A survey of the results of coagulation studies of blood samples obtained from peripheral Saline lock and Heparin lock catheter and comparing it with direct blood sample Beheshti Z Ashraf Pour M Haji Ahmadi M 1 10 2004 6 4 17 22 31 05 2014 Background and Objective: Healthcare staffs want to use an accurate method for taking blood sample. To prevent frequent venipunctures in patients who require a routine blood sampling for controlling PT and PTT, 2 methods of Saline lock catheter flushing and Heparin lock catheter flushing were compared to each other. Methods: This study was performed on 60 patients. They were divided into 2 equal groups. Vein catheter for 1 group was flushed by heparinized Saline and for the other group was flushed by saline. 2 blood samples were obtained, one from vein by needle and the other from catheter after 2 ml blood aspiration. PT and PTT tests were performed by coagulometer. Data were analyzed and compared by T-test and paired T-test. Findings: It was not seen a significant difference for PT in 2 methods of Saline lock and Heparin lock with direct method. There was a significant difference for PTT between Heparin lock and direct method (P=0.000) and also between Heparin lock and Saline lock (P=0.006). Conclusion: According to the results, vein Saline lock catheter in comparison to heparinized catheter is a reliable and proper method for coagulation studies. With regard to advantages of normal saline in comparison to heparin, it is suggested that saline to be used for flushing peripheral cannulas. 2665 Biochemical The effect of missing data in growth curves Haji Ahmadi M Ayatollahi M.T Behboodian J 1 10 2004 6 4 23 29 31 05 2014 Background and Objective: Applying the growth curve is the most powerful way for monitoring the growth in children and through this method it would be possible to recognize in time the deviation from the natural growth pattern in children. Falling the data and missing values are general problems in analyzing the growth longitudinal data. Therefore, it is important that by computing the missing values, the data should be completed and directed towards the proper path for analysis. Methods: This 2 year longitudinal study was done on 317 infants (153 boys and 164 girls) in Shiraz during 1996. The information related to growth (Weight, height, round the head, round the arm, and round the chest) at the birth time were collected and 11 visits from the infants’ living houses were done. In order to influence the missing values on the growth charts, 4 methods (Ignoring the missing values, general and individual models of growth curve and multiple imputation) were considered to study. Mean, 3rd, 50th, 97th centiles of raw and smooth weight were computed in boys and the smooth growth charts of their weight were determined and compared based on the four methods. Findings: There was no noticeable difference in the boys’ mean weight at age under study according to growth curve methods and multiple imputation while missing values were ignored. However, the smooth growth charts showed that applying the individual growth curve model (Second level) and multiple imputation causes the noticeable difference between the values of 3rd, 97th centiles and the traditional analysis (Ignoring missing values). Conclusion: Regarding the existence of missing values in growth longitudinal studies, ignoring the missing values for analyzing is not acceptable. Applying the growth curve model method could be considered important in making desirable the analysis and the proper growth path. 2666 Biochemical Determination of required space for canine and premolar eruption in mixed dentition using mesiodistal width of mandibular incisors Arash V Mir Kazemi A 1 10 2004 6 4 30 33 31 05 2014 Background and Objective: Determination of the relation between periphery of dental arch and teeth size which mostly done before eruption of some permanent teeth, is of important levels of diagnosis in mixed dentition. Determination of this relationship is not possible without prediction and distinct evaluation of unerupted permanent mesiodistal width (Permanent canines and premolars). The aim of this study was to evaluate the required space for eruption of canine and premolars using total width of lower incisor in Iranian people by Tanaka & Johnston method. Methods: In this study mesiodistal width of permanent first and second premolar and canine in each quadrant also of 4 lower incisors were measured in 604 dental casts of under 20 year old people (In Mazandaran) using Koulis with accuracy of 0.02 millimeter and final measurements were illustrated in a table. Needed teeth for measurement were completely erupted, caries free and without fracture or restoration. Findings: The mean of mesiodistal width of lower incisors, total mesiodistal width of upper and lower canine and premolars and a and b indexes for Tanaka and Johnston equation (Y=aX+b), a=0.167, b=19.387 for mandible and a=0.195, b=18.723 for maxilla have been calculated. Meanwhile X is the sum of lower incisor mesiodistal width and Y is the sum of canine and premolar mesiodistal width in one side. Conclusion: In present study, equation of Tanaka and Johnston is recommended. So its dependence on race is obvious. 2667 Biochemical Left ventricular systolic dysfunction among nocturnal myocardial infarctions Ghaemian A Naderi N Zand Parsa AF 1 10 2004 6 4 34 38 31 05 2014 Background and Objective: Diurnal variation in the onset of acute myocardial infarction with a peak in the morning has been demonstrated. Few data are available concerning about the left ventricular systolic dysfunction according to the infarct time. Regarding importance of onset of heart failure following acute myocardial infarction, this study was done to survey the effect of the time of acute myocardial infarction on left ventricular systolic dysfunction. Methods: This prospective study was performed on 100 patients with acute myocardial infarction (AMI). Left ventricular systolic dysfunction (LVSD) was defined by echocardiography when ejection fraction (EF) was less than 55%. Findings: Of these patients, 72 had EF<55% and 37% of them experienced myocardial infarction between 6 am and 5:59 pm (Group 1) and 63 experienced their AMI between 6 pm and 5:59 am (Group 2). Left ventricular EF less than 55% occurred in 22 (59.5%) patients of group 1 compared with 50 (79.4%) in group 2 (P=0.04). Mild, moderate and severe LVSD were also more frequent in group 2 patients (P=0.01). Conclusion: The risk of mild, moderate and severe left ventricular systolic dysfunction after AMI is higher among infarctions that begin at night. 2668 Biochemical Response to Hepatitis B vaccination in health care workers of Yahyanejad Hospital, Babol, 2002 Kashi Fard M Hassanjani Roshan MR 1 10 2004 6 4 39 42 31 05 2014 Background and Objective: Worldwide Hepatitis B virus (HBV) infection is a major source of morbidity and mortality, with an estimated 300 million persons infected. Health care workers are at higher risk for HBV infection. Vaccination and then evaluation of their antibody response is important for viral control. This study was done to evaluate anti-HBS response following HBV vaccination in health care workers of Yahyanejad hospital in Babol. Methods: This descriptive and analytical study was performed on 220 health care workers of Yahyanejad hospital. Anti-HBS and anti-HBC positive cases were excluded from the study. HBsAb was measured in all cases 3 months after the latest vaccination. Findings: From 204 cases, non-responders were seen in 52 (25%) cases. Poor and good responders were seen in 18 (41.9%) and 25 (22.9%) males and in 25 (58.1%) and 84 (77.1%) females, respectively (P<0.05). Totally, 74.5% were antibody positive. Conclusion: Health care workers are at high risk group for HBV infection. The non-responders need additional vaccination. 2669 Biochemical Hydatid cyst components effect in prevention of Hymenolepis Nana infection in rat Yousefi MR Sharif M Izadi J Ajami GH Khalilian AR 1 10 2004 6 4 43 48 31 05 2014 Background and Objective: Regarding prospective effects and cross immunity between some species of parasites, obtained antigens of a parasite can be used to prevent other parasitic diseases and adjuvant of this effect can be increased. The aim of this study was to evaluate the effect of hydatid cyst components in prevention of H.Nana in rat. Methods: 40 rats with the same sex and age were selected after gaining confidence in nonexistence of any parasitic disease. They were divided into 2 groups and each group were divided into 4 subgroups. In one group, antigen of fluid, protoscolex, germinal layer were injected respectively and in the other group, these antigenic materials were used with an adjuvant. Control group was considered for both groups. The immunization was performed by multiple injection. One week after injection of antigenic materials, H.Nana eggs were received orally by rats. After observation of H.Nana in stool of rats in control group, blood sample was taken from all rats. Different biochemical tests and immunological and parasitological tests were performed on sera and stool samples, respectively. Findings: Different protein measurements especially gammaglobulin indicated that membrane of hydatid cyst had the strongest antigenic effect in comparison to 2 other parts of cyst and hydatid cyst fluid showed the weakest one. Conclusion: Stool test in control group and exposing egg showed that this group was infected. It was not seen any exposing egg in rats that immunized with different hydatid cyst components. Statistical and biochemical results demonstrated that this effect increased by using adjuvant with different hydatid cyst components and its results showed that immune effect would be increased after injection of adjuvant. 2670 Biochemical Prevalence of HBV DNA in anti-HBe+ chronic HBV carriers in Babol, 2002 Hassanjani Roshan MR Soleimani MJ Asghar Zadeh Ahmadi A 1 10 2004 6 4 49 53 31 05 2014 Background and Objective: One of the most important mutations, which occur in hepatitis B virus, is precore mutant. This mutation is associated with highly productive infection in anti-HBe+ chronic HBV carriers. These chronic HBV carriers are predisposed to developing active hepatitis. The aim of this study was to determine the prevalence of HBV DNA in chronic anti-HBe+ HBV carriers in Babol. Methods: This cross-sectional study was conducted on patients with anti-HBe+ chronic HBV carriers in Babol during 2002. In all cases, HBV DNA was assayed by PCR. Prevalence of HBV DNA is males, females and different age groups were compared with X² test. Findings: Of 257 cases of anti-HBe+ (Mean age±SD, 32.3±11.2 years), HBV DNA was positive in 222 (86.4%) cases. HBV DNA was positive in 136 (87.2%) of 156 males and 86 (85.15%) of 101 female cases (P=0.7). There was no significant difference in prevalence of HBV DNA between age groups (P=0.07). Conclusion: These research show that the high prevalence of HBV precore mutants in this region. Follow up of these individuals are necessary. 2671 Biochemical Dissemination: A critical activity in knowledge management Asef Zadeh , S Piri Z 1 10 2004 6 4 54 62 31 05 2014 Background and Objective: Today countries are classified according to their abilities to knowledge generation and utilization. Knowledge management has become a critical issue in organizations and knowledge dissemination is an important activity in knowledge management. The aim of this research was to study theoretical issues about dissemination of information and knowledge and features of effective dissemination for utilizing of knowledge products. Methods: This systematic study was conducted using library resources in the libraries of Iran university of medical sciences, the internet search engines (Google, Mamma), databases (PubMed, Science@direct) from 1990 to 2003 and the website of national center for the dissemination of disability research (NCDDR). Findings: Activities about understanding of knowledge concept and focus on dissemination have been considered as third wave since 1980. Researches conducted about dissemination had 2 main directions: Who were seeking the most appropriate approach for disseminating and who were seeing dissemination issue from the view of users. There are 5 key components for effective dissemination: Source, content, context, media and the user. Effective dissemination needs to appropriate interaction among these factors. Dissemination is not a liner phenomenon Rather there is a close relationship and collaboration among generation, dissemination and utilization. There are 4 kinds of knowledge and each one needs to different way of dissemination. Conclusion: As information utilization is the aim of dissemination, therefore 5 key components in dissemination, i.e. source, content, context, media and user must have some necessary characteristics in addition to coordination. Any organization, in particular research organizations, who thinks of competitive advantages in today’s world, must develop a proper plan for its knowledge dissemination considering the features of effective dissemination and user participation. 2672 Biochemical Effect of repairing and medication on treatment of perforation of peptic ulcer Hashemi R Savad Koohi SH Mansoori P 1 10 2004 6 4 63 65 31 05 2014 Background and Objective: Perforation of peptic ulcer is a common complication that its treatment needs several surgical methods (Repairing, vagotomy and anterectomy). These methods in spite of technical difficulties and complications do not exclude the helicobacter pylori. This study was performed to evaluate the efficacy of simple repairing with anti-acid and anti-helicobacter agents in treatment of perforation of peptic ulcer. Case: This study was performed on 10 cases with perforated peptic ulcer. After simple closure of perforation, all patients were treated by metronidazole, amoxicillin, cimetidine, bismuth, omeprazole. After 6 weeks of treatment, an endoscopic examination was performed. All patients were treated completely by this method. Conclusion: According to the results, simple surgical repairing of perforation in addition to administration of anti-acid and anti-helicobacter treatment is sufficient for therapy of perforation of peptic ulcers. 2673 Biochemical Multiple brain infarctions and aneurysm of interatrial septum: Case report Saravi M, Hojati SMM 1 10 2004 6 4 66 70 31 05 2014 Background and Objective: Previous reports have described an association between atrial septal aneurysm and cerebral embolic events. We report this patient for clinical presentation of multi-infarction and role of transesophageal echocardiography in diagnosis of this condition. Case: A 40-year-old man was admitted in hospital because of sudden right hemiparesis, which repeated after three weeks with Wernicke dysphasia and after that degrees of apathy. Brain CT scan revealed some hypodense areas. Ultrasound study failed detect any thrombosis or stenosis in carotid, vertebral and basilar arteries. MRI was in favor of vascular events. No arrhythmia was found in holter ECG. Transthoracic and transesophageal echocardiography were in favor of atrial septal aneurysm without patent foramen ovale or any thrombus. Warfarin was started and there was no recurrence during follow up. Conclusion: Inter-atrial septal aneurysm is probably an important factor for stroke. Embolic event is due to migration of in situ thrombosis or transition through it. Marked mobility and thickness of aneurysm may also increase the risk of embolization. Transesophageal echocardiography is a helpful tool in the diagnosis and management of patients with stroke and this anomaly. 2674 Biochemical Coexistence of gout and Sarcoidosis: Case report Heidari B Bijani KH Shafigh E 1 10 2004 6 4 71 74 31 05 2014 Background and Objective: Sarcoidosis (Sa), a multisystem disease of unknown etiology presents protean manifestations. Triad of Sa-gout-psoriasis has been reported in a few patients but coexistence of Sa and gout has not been reported. Case: A 36-year-old man presented with acute polyarthritis, bilateral hilar adenopathies and erythema nodosum. Diagnosis of acute gout was established with regard to clinical findings, refusing other causes of acute arthritis and identification of monourate crystals from synovial fluids. Diagnosis of Sa was confirmed by extensive exploration including CT scanning of thorax, bronchoscopy and biopsy of epitrochlear adenopathies. Conclusion: Development of gout may be due to hyperuricemia (Caused by granolumatous tissue destruction) or release of inflammatory cytokines from granolumatous tissue destruction of Sa.