Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Seroprevalence of Helicobacter pylori infection in adolescents and adults in Babol
7
12
FA
M
Zamani
Cancer Research Center, Health Research Center, Babol University of Medical Sciences, Babol, I.R.Iran
A
Vahedi
Student Research Committee, Babol University of Medical Sciences, Babol, I.R.Iran
V
Zamani
Deputy of Health, Babol University of Medical Sciences, Babol, I.R.Iran
A
Bijani
Research Center for Social Factors Affecting Health, Health Research Center, Babol University of Medical Sciences, Babol, I.R.Iran
J
Shokri-Shirvani
Research Center for Infectious Diseases and Tropical Medicine, Health Research Center, Babol University of Medical Sciences, Babol, I.R.Iran
-BACKGROUND AND OBJECTIVE: Helicobacter pylori is an important causative factor for chronic gastritis, peptic ulcer and gastric cancer. Based on the reports, about half of the world population are infected with this bacterium. The present study was designed with the purpose of investigation of H. pylori infection rate in adolescents and adults of Amirkola in Babol
METHODS: This cross-sectional study was performed on individuals who referred to the health and treatment center of Amirkola, Babol, for the premarital counseling during December 2015 and January 2016. The blood sample of subjects was assessed for H. pylori status using enzyme-linked immunosorbent assay. The demographic data were collected.
FINDINGS: Of 207 participants, 100 (48.3%) were male and others were female. The mean age was 25.35±5.82 (14-46) years. Rate of H. pylori was 43%, seen more frequently in the age group 21-25 years (37.2%). The infection rate was 49% and 37.4% in males and females, respectively, but the difference was not significant. The age group more than 30 was associated with increased risk of infection (odds ratio [OR]=5.25, 95% confidence interval [CI]: 1.86-14.78). There was also a significant correlation between the infection and level of education (p=0.041) and higher diploma was inversely associated with the infection (OR=0.4, 95% CI: 0.18-0.91). No significant relation was found between the infection and blood groups and residency.
CONCLUSION: The results of study indicated a considerable rate of H. pylori infection in adolescents and adults of Amirkola. Also, it was determined that higher education can have a protective role against H. pylori infection
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Biosynthesis of Iron Nano-Particles by Bacillus Megaterium and Its Anti-Bacterial Properties
13
19
FA
S
Ghani
Young Researchers and Researchers Club, Arak Branch, Islamic Azad University, Arak, I.R.Iran
B
Rafiee
Young Researchers and Researchers Club, Arak Branch, Islamic Azad University, Arak, I.R.Iran
D
Sadeghi
Biology Research Center, Faculty of Science & Basic Sciences, Imam Hossein University of Technology, Tehran, I.R.Iran
M
Ahsani
Department of Horticulture, Faculty of Agriculture, Arak University, Arak, I.R.Iran
BACKGROUND AND OBJECTIVE: According to the environmental pollution caused by chemical and physical methods of synthesis of nanoparticles and their incompatibility in medical, The aim of this study is biosynthesis of iron nano particles and its antibacterial activity.
METHODS: In this experimental study, Bacillus megaterium PTCC1656 was cultured in nutrient broth medium, Then bacterial suspension was combined at a ratio of 1:1 with 0.1 M iron nitrate.Synthesis of iron nanoparticles confirmed with Uv-vis, XRD and SEM techniques. Antibacterial properties of nanoparticles were evaluated by disk diffusion method and dilution tube MIC (Minimum inhibitory concentration) on standard strains of Staphylococcus aureus PTCC 1431, Bacillus cereus 1015 PTCC and Escherichia coli PTCC 1399, Pseudomonas aeruginosa PTCC 1571.
FINDINGS: The peak of absorbtion for iron nanoparticle was 220 nm. Iron nanoparticles was cuboid shape. The average size of the nanoparticles was 40-60 nm. MIC was measured for Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa 0.031, 0.031, 0.0078, 0.015 mg/ml respectively.
CONCLUSION: Results showed iron nanoparticle have antibacterial effect on pathogenic bacteria. The highest inhibition zone diameter by biological iron nitrate nanoparticles was observed for E. coli and the minimum diameter of inhibition was observed for Bacillus cereus
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Antibody Drug Conjugates for Cancer Therapy
20
27
FA
S M
Gheibi hayat
Department of Biotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R.Iran
AH
Sahebkar
Biotechnology Research Center, Mashhad University of Medical Sciences, I.R.Iran
BACKGROUND AND OBJECTIVE: In recent decades, the use of antibody drugs conjugates (ADCs) generated promise for the treatment of cancer. In this type of treatment, a monoclonal antibody against a cancer specific antigen is used, and a cytotoxic drug is attached to the antibody via a linker. This smart drug delivery system also named Armed Antibody. In this review, important factors for the design and performance of an ADC are described.
METHODS: Search by the keywords “Antibody Drug Conjugate” in databases Pubmed, Scopus and Web of Science were done and then 58 related articles that published in 2000-2017 were selected.
FINDINGS: To develop a suitable ADC different parameters should be considered. The choice of the type of antibody, drug and linker should be based on different factors to achieve an ADC with optimal performance. far, more than 671 clinical trials have been registered in Clinical Trial Database registry (www.clinicaltrials.gov) using the keyword ‘antibody drug conjugate’, but only three drugs with trade names, Mylotarg, Adcetris® and Kadcyla® have received FDA approve however the production of Mylotarg is stopped due to lethal effects.
CONCLUSION: Cancer treatment by traditional methods due to the effects of chemotherapy drugs on normal cells caused adverse effects but the use of ADCs can induces an apoptosis effects on tumor cells by targeted drug delivery
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Antimicrobial Resistance Patterns and Frequency of Extended-Spectrum Beta-Lactamase Genes among Acinetobacter Baumannii
28
34
FA
F
Ahmadikiya
Yazd University of Medical Sciences, International Branch, Yazd, I.R.Iran
A
Mosadegh
Department of Microbiology, Faculty of Medicine, Yazd University of Medical Sciences, Yazd, I.R.Iran
M
Moradi
Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, I.R.Iran
H
Hossieni-Nave
Department of Microbiology, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, I.R.Iran
BACKGROUND AND OBJECTIVE: Acinetobacter baumannii is an opportunistic pathogen that is resistant to many antibiotics including beta-lactams. Production of β-lactamases is the main mechanism of β-lactam resistance in A. baumannii. The aim of the present study was to determine the antimicrobial susceptibility profile and frequency of ESBL genes in clinical isolates of A. baumannii in hospitals in Kerman, Iran.
METHODS: In this cross-sectional study 102 isolates of Acinetobacter species were collected from clinical specimens, including, respiratory secretions, urine, blood culture and body fluid. For confirming A. baumannii Polymerase chain reaction (PCR) was used to identify blaOXA-51 gene. Antimicrobial susceptibility test was performed using the disk diffusion method. PCR technique was carried out for the detection of blaCTX-M, blaSHV, blaTEM, blaPER and blaVEB genes.
FINDINGS: blaOXA-51 gene was detected in 95 (93/1%) isolates. All of the isolates were resistant to Cefepime and Cefotaxime. Almost all of them were resistant to Imipenem, Meropenem, Gentamicin, Amikacin, Aztreonam, Ciprofloxacin, Ceftazidim, Levofloxacin, Tetracycline and Piperacillin-Tazobactam. The rates of susceptibility to Polymyxin-B and Tigecycline were 61/1% and 23/2%, respectively. The frequency of, blaSHV, blaTEM, blaCTX-M and blaVEB genes were 44 (46.3%), 30 (31.6%), 30 (31.6%), and 13 (13.7%), respectively. None of the isolates were carried blaPER gene.
CONCLUSION: Because of the high rate of ESBLs producing A. baumannii isolates detected in this study, effective infection control strategy should be performed.
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Introduction and Optimization of a Dietary Model for Inducing Hyperlipidemia in Rats
35
41
FA
A
Amirabadizadeh
Department of Biology, Islamic Azad University, Kazerun Branch, Kazerun, I.R.Iran
M
Foaddodini
Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, I.R.Iran
S
Khatamsaz
Department of Biology, Islamic Azad University, Kazerun Branch, Kazerun, I.R.Iran
M
Mokhtari
Department of Biology, Islamic Azad University, Kazerun Branch, Kazerun, I.R.Iran
M
Shariati
Department of Biology, Islamic Azad University, Kazerun Branch, Kazerun, I.R.Iran
M
Hosseini
Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjnad, I.R.Iran
F
Hajipour
Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, I.R.Iran
BACKGROUND AND OBJECTIVE: Inducing hyperlipidemia in laboratory animals through diet is a good way to study metabolic disorders. This study was conducted to provide an effective and accessible diet for generating hyperlipidemia and non-alcoholic fatty liver models in rats.
METHODS: In this experimental study, 40 male Wistar rats (180-200 g) were divided into 5 equal groups including 2 control groups receiving regular diet for 4 (C1) or 8 weeks (C2), and 3 experimental groups receiving high fat diet along with 0.2% (E1) or 0.1% thiouracil (E2) for 8 and 4 weeks (E3). Finally, the concentration of total cholesterol (TC), LDL, HDL, and triglyceride (TG) was measured and the fat accumulation in the liver tissue was measured quantitatively.
FINDINGS: All experimental groups had significantly higher TC, TG, LDL and lower HDL compared to control (p<0.0001). The cholesterol level was significantly higher in E1 (642.66±133.01), E2 (848.16±146.17) and E3 (406.83±116.28) groups, compared with the C1 (64.87±16.10) and C2 (76.83±11.37) groups (p<0.0001). The degree of fat accumulation in the groups E1 (3.70±0.34), E2 (3.45±0.32) and E3 (2.83±0.25) was significantly higher than the groups C1 (0.25±0.01) and C2 (0.33±0.03) (p <0.0001).
CONCLUSION: The high-fat diet introduced in this study can cause hyperlipidemia and non-alcoholic fatty liver rats within 4 weeks
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
An Evaluation of New Indices of Postural Sway in Patients with Knee Osteoarthritis
42
49
FA
SM
Mohsenipour
Department of Physiotherapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R.Iran
R
Ravanbod
Department of Physiotherapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R.Iran
G
Torkaman
Department of Physiotherapy, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, I.R.Iran
N
Bayat
Department of Internal Medicine, Faculty of Medical Sciences, Baqiyatallah University of Medical Sciences, Tehran, I.R.Iran
BACKGROUND AND OBJECTIVE: Reduced joint proprioception plays an important role in the initiation and progression of the knee osteoarthritis. The present study aims to evaluate postural sway in three different positions and analyze its trends using relative indices.
METHODS: This cross-sectional study was conducted among 34 patients with knee osteoarthritis and 12 healthy people using selective sampling and convenience sampling. In order to evaluate the static equilibrium indices, patients were asked to sit on the force plate in three positions; Comfort Double Leg Standing (CDLS), Romberg Standing (RS) and Near Tandem Standing (NTS). The relative values of and were used to determine the changes in postural sway in RS and NTS positions relative to CDLS.
FINDINGS: In CDLS position, the range and the standard deviation of lateral position in patients (0.019 ± 0.010 and 0.003±0.001, respectively) was lower than healthy people (0.051 ± 0.039 and 0.012 ± 0.010, respectively). In this position, the displacement range in the anterior-posterior direction in patients (0.110 ± 0.029) was also lower than healthy people (0.130 ± 0.027). In RS position, standard deviation and the velocity of center of pressure displacement in the patients group (0.016 ± 0.006 and 0.012 ± 0.004, respectively) was significantly higher than healthy people (0.012 ± 0.003 and 0.009 ± 0.002, respectively). On the contrary, the ratio of and values was higher in patients group.
CONCLUSION: Results of the study demonstrated that patients with more difficult positions (RS, NTS) have more sways than those with CDLS, especially in the lateral direction. Therefore, balancing and strengthening exercises are especially important in the lateral direction and in challenging situations.
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Effect of Aqueous Extract of Artemisia absinthium L. on Sex Hormones, Inflammatory Cytokines and Oxidative Stress Indices of Ovarian Tissue in Polycystic Ovary Syndrome Rat Model
50
56
FA
S.D
Sadoughi
Young and Elite Research Club, Mashhad Unit, Islamic Azad University, Mashhad, I.R.Iran
R.
Rahbarian
Department of Biology, Faculty of Science, Payame Noor University of Tehran, Tehran, I.R.Iran
N.
Jahani
Department of Biology, Faculty of Science, Payame Noor University of Tehran, Tehran, I.R.Iran
S.A.
Shazdeh
Department of Biology, Faculty of Science, Payame Noor University of Tehran, Tehran, I.R.Iran
S.
Hossein Zadeh Saljoughi
Department of Biology, Faculty of Science, Payame Noor University of Tehran, Tehran, I.R.Iran
M.
Daee
Department of Biology, Faculty of Science, Payame Noor University of Tehran, Tehran, I.R.Iran
BACKGROUND AND OBJECTIVE: Hormonal disorders along with oxidative stress and inflammation in ovarian tissue lead to anovulation in polycystic ovary syndrome (PCOS) patients. Considering antioxidant effect of Artemisia absinthium, purpose of this study is to determine the effect of aqueous extract of Artemisia absinthium on serum levels of sex hormones, inflammatory cytokines and oxidative stress indices of ovarian tissue in polycystic ovary syndrome rat model.
METHODS: In this study, 32 female Wistar rats were divided into 4 equal groups: groups of control, PCOS control and two groups with PCOS which are under treatment through aqueous extract of Artemisia absinthium (100 and 200 mg/kg). Polycystic ovarian syndrome was induced by single intramuscular injection of estradiol valerate (4mg/kg). Aqueous extract of Artemisia absinthium was intraperitoneal injection to PCOS treated groups, for 24 days. At the end of treatment period, serum levels of LH, FSH, Estradiol, Testosterone, TNF-α, IL-1β and IL-6 and levels of SOD, CAT, GPX enzymes and level of MDA in ovarian tissue were measured through ELISA method.
FINDINGS: Compared to PCOS control group (LH: 14.30±2.52, FSH: 2.35±0.28, Estradiol: 17.61±2.44, Testosterone: 10.29±1.56, TNF-α: 178.65±4.35, IL-1β: 121.52±5.17, IL-6: 162.28±5.83, SOD: 20.51±1.84, CAT: 64.42±3.70, GPX: 35.15±2.88, MDA: 87.32±3.40), MDA tissue level (55.46±4.73), serum levels of LH (8.26±1.36), Estradiol (7.76±1.55), Testosterone (6.40±1.04) and TNF-α (115.35±5.83), IL-1β (70.25±5.74) and IL-6 (89.15±4.52) cytokines in group under treatment with 200 mg/kg aqueous extract of Artemisia absinthium significantly decreased (p=0.008) and serum level of FSH (7.52±1.21) and levels of SOD (71.58±5.19), CAT (128.30±5.11) and GPX (88.21±5.51) antioxidant enzymes of ovarian tissue significantly increased (p=0.005).
CONCLUSION: Aqueous extract of Artemisia absinthium by decreasing the serum levels of inflammatory cytokines and increasing the activity of the ovary tissue antioxidant enzymes has a favorable effect on the improvement of hormonal parameters in rats with polycystic ovary syndrome
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Optimization of Image Quality and Patient Dose in Digital Radiography of the Chest
57
62
FA
R
Paydar
Department of Radiation Sciences, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, I.R.Iran
SH
Mousavie Anijdan
Department of Radiation Technology, Allied Medicine Faculty, Babol University of Medical Sciences, Babol, I.R.Iran
A
Khorrami Moghaddam
Department of Radiology, Allied Medicine Faculty, Mazandaran University of Medical Sciences, Sari, I.R.Iran
I
Mohammadi
Department of Basic Sciences, Faculty of Medicine, Sari Branch, Islamic Azad University, Sari, I.R.Iran
R
Reiazi
Department of Medical Physics, School of Medicine, Iran University of Medical Sciences, Tehran, I.R.Iran
BACKGROUND AND OBJECTIVE: Digital systems have been replacing with screen-film analogue systems in diagnostic radiology departments, rapidly. Despite the differences in the properties of new x-ray imaging detectors, the same radiographic protocols that had been used for radiographic film-screen are used for digital imaging systems, without any review yet. In this study, the image quality and the patient dose in digital imaging of the chest are evaluated and optimized.
METHODS: Two digital radiography machines from two separated hospitals (Imam Khomeini and Bu Ali Hospitals-Sari) have been used in this experimental research. Imaging and dose measurement are carried out at different source to phantom distances and kilo-voltages. For measurement of the image quality, a contrast-detail radiography (CDRAD) phantom is used. For evaluation of optimization, the Inverse Image Quality Figure per patient dose squared (IQFinv/E2) is used.
FINDINGS: Evaluation of measured data for optimization shows that for both of these two digital radiography machines, despite of increasing in patent dose, with reducing of kilo-voltage, the IQFinv/E2 is increased. The maximum values of this parameter for Imam Khomeini and Bu Ali Hospitals are measured 0.0180 and 0.0083, respectively.
CONCLUSION: The results of this study indicate that despite the traditional notion of using higher kilo-voltages for chest radiography, with increasing kilo-voltage, the ratio of image quality per patient dose is reduced. So, for optimization of chest radiography, as much as possible the kilo-voltage should be reduced based on the size of patient and clinical purpose.
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
The Effect of Dietary Constituents on Regulation of Epigenetic Changes in Cancer
63
71
FA
S
Barghi
Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Science, Tehran, I.R.Iran
M
Amiri
Department of Medical Laboratory Sciences, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Science, Tehran, I.R.Iran
H
Hajipur
Department of Reproductive Biology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, I.R.Iran
S
Namaki
Department of Immunology, Faculty of Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, I.R.Iran
BACKGROUND AND OBJECTIVE: The term “epigenetic” refers to all non-heritable and reversible changes in the expression of a gene that does not cause a change in the DNA sequence. The most important epigenetic mechanisms associated with gene expression include DNA methylation, histone modifications, and suppression of gene expression with RNA. Considering the reversibility of epigenetic changes, it seems that this feature can be influenced by dietary constituents and thus, we can prevent the spread of certain cancers by controlling the diet. The purpose of this study is to investigate the effects of food on the prevention of common cancers and the mechanisms involved in cellular activities based on recent studies and the compilation of their results.
METHODS: In this review article, we searched Pubmed and Elsevier databases using certain keywords such as “epigenetics”, “cancer” and “nutrition” and articles related to the effects of epigenetics on cancer and dietary constituents were evaluated.
FINDINGS: Of 439 studies found in the search engines between 1997 and 2016, 64 articles were selected and their results indicated that many of the active components in the diet will inhibit the incidence of cancer through DNA methylation mechanisms, histone modifications, and miRNA.
CONCLUSION: The anticancer effect of the active compounds in the diet on specific epigenetic changes can be used as a special and unidentified mechanism for preventing cancer
Journal of Babol University Of Medical Sciences
Journal of Babol University of Medical Sciences
1561-4107
19
7
2017
7
1
Investigating the Causes of Heart Failure based on Persian Medicine Point of View
72
78
FA
R
Ghods
Medical History Studies Institute, Islamic Medicine & Supplement, Faculty of Traditional Medicine, Iran University of Medical Sciences, Tehran, I.R.Iran
R
Moeini
Traditional Medicine and Medical History Research Center, Health Research Center, Babol University of Medical Sciences, Babol, I.R.Iran
N
Gorji
Traditional Medicine and Medical History Research Center, Health Research Center, Babol University of Medical Sciences, Babol, I.R.Iran
F
Ghorbani
Faculty of Traditional Medicine, Tehran University of Medical Sciences, Babol, I.R.Iran
BACKGROUND AND OBJECTIVE: Nowadays, heart failure is one of the most common diseases and is the cause of death in a high percentage of the population. Despite all the progress made, researches continue for achieving a better understanding of its causes. The present study aims to evaluate the causes of heart failure from Persian medicine (PM) point of view.
METHODS: In this review article, first PM books with references to causes of heart failure and weakness such as Al Qanun Fi Al-Tibb (The Canon of Medicine) and Exir-e-azam were reviewed and compared with modern medical findings in Braunwald's Heart Disease, Harrison's Principles of Internal Medicine and Cecil medicine books as well as online databases including PubMed, Science direct, Scopus and Google scholar.
FINDINGS: Reviewing the documentation revealed that, according to definitions and symptoms, heart failure is the equivalent of the term “heart weakness” in PM. Accordingly, results of the study demonstrated that according to PM, causes such as Sue-e-Mizaj, obstruction, tissue detachment, mental disorders, direct and indirect excessive disposal of body essential materials may underlie the heart’s inability to meet the needs of the organs.
CONCLUSION: Several factors are mentioned as the cause of heart failure or heart weakness in PM, only a few of which are considered in modern medicine. However, some of these less considered theories can be used as new assumptions to prevent and control this disease