fa بررسی ژنهای بتالاکتاماز طیف گسترده blaCTX-Mbla SHV, bla TEMدر سویه‌های اشریشیاکلی جدا شده از عفونتهای ادراری و مقاومت آنتی‌بیوتیکی آنها میکروبیولوژی Microbiology تحقیقی Research <p dir="rtl" style="margin: 0cm 0cm 0.0001pt text-align: right line-height: 15.6pt direction: rtl unicode-bidi: embed"><font face="times new roman, times, serif"><strong><span lang="AR-SA" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">سابقه و هدف</span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></span></strong><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">آنزیم</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">‌</span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">های بتالاکتامازهای طیف گسترده، هیدرولیز کننده پنی‌سلین</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">‌</span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ها و سفالوسپورین</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">‌</span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ها می‌باشند. هدف از این بررسی تعیین فراوانی سویه­های اشریشیاکلی</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">‌<span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">تولیدکننده ژنهای بتالاکتامازهای</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> CTX-M</span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">،‌</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">TEM</span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">،</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">SHV </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">با روش</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> Multiplex PCR </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">و ارتباط آنها با ایجاد مقاومت آنتی بیوتیکی در سویه‌های اشریشیاکلی</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">‌</span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">است</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span></span><span dir="ltr" style="font-size: 9pt"><u1:p><!--stripped--><!--stripped--></u1:p></span></font></p> <p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 15.6pt direction: rtl unicode-bidi: embed text-align: start"><font face="times new roman, times, serif"><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مواد و روشها</span></span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></span></strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">در این مطالعه مقطعی، تعداد 55 سویه اشریشیاکلی با استفاده از محیط کشت</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> EMB </span></span><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">آگار و کروم آگار</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> E.coli </span></span><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">از نمونه‌های ادراری جداسازی و بعد از تایید توسط آزمونهای بیوشیمیایی، آزمون تعیین حساسیت آنتی‌بیوتیکی با روش دیسک دیفیوژن</span></span><span class="apple-converted-space"><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">(DiskDiffusion)<span class="apple-converted-space"> </span></span></span><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">و با آنتی­بیوتیکهایی انتخابی مطابق با د</span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ستورالعمل</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> CLSI</span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">، انجام شد. حضور ژنهای</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> ^blaCTX-M </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">،</span></span>^{<span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">bla </span></span>}<span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">TEM </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">،</span></span>^{<span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">bla</span></span>}<span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">SHV </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">با استفاده از پرایمرهای اختصاصی توسط</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">چندگانه ­ای</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> PCR </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">انجام گردید</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span></span><span lang="AR-SA" style="font-size: 9pt"><u1:p><!--stripped--><!--stripped--></u1:p></span></font></p> <p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 15.6pt direction: rtl unicode-bidi: embed text-align: start"><font face="times new roman, times, serif"><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">یافته</span></span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">­</span></span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ها</span></span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></span></strong><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">بیشترین میزان مقاومت به آنتی­بیوتیکهای پنی­سیلین و اریترومایسین به ترتیب با 96% و 94/5% فراوانی و بیشترین میزان حساسیت به آنتی­بیوتیکهای سیپروفلوکساسین و ایمی­پنم با 67/2% فراوانی گزارش شد. از 55 نمونه مورد آزمایش 26 نمونه 47/26</span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">% </span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">واجد ژن</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> TEM </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">بوده و در 41 نمونه 74/54% ژن</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> CTX-M </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">شناسایی شد همچنین در 32/72% از نمونه­</span></span><span class="apple-converted-space"><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">ها هر دو ژن</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> TEM </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">و</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> CTX-M </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">به طور همزمان شناسایی و در 6 نمونه 10/9% هیچ یک از ژنهای‌ مذکور شناسایی نگردید. همچنین ژن</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">SHV </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">در هیچ یک از نمونه­ها شناسایی نشد</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span></span><span lang="AR-SA" style="font-size: 9pt"><u1:p><!--stripped--><!--stripped--></u1:p></span></font></p> <p dir="rtl" style="margin: 0cm 0cm 0.0001pt line-height: 15.6pt direction: rtl unicode-bidi: embed text-align: start"><font face="times new roman, times, serif"><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نتیجه</span></span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></span></strong><strong><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span lang="AR-SA" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">گیری</span></span></strong><strong><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></strong><span class="apple-converted-space"><b><span dir="ltr" style="font-size: 9pt color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> </span></b></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نتایج آزمایشات نشان می‌دهد که تولید آنزیم‌های بتالاکتاماز وسیع ­الطیف در 70% از سویه</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">‌</span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">های مورد مطالعه مشاهده شد که جهت جلوگیریاز شیوع سویه‌های اشریشیاکلی تولیدکننده</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"> ESBL </span></span><span lang="AR-SA" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">باید مراقبت‌های دقیق پزشکی و استفاده صحیح و به موقع از آنتی‌بیوتیک‌های مناسب صورت پذیرد</span></span><span dir="ltr" style="font-size: 9pt background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial"><span style="background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span></span><span lang="AR-SA" style="font-size: 9pt"><!--stripped--><!--stripped--></span></font></p> <p dir="rtl" style="margin: 0cm 0cm 0.0001pt text-align: right line-height: 15.6pt direction: rtl unicode-bidi: embed"></p> <p class="MsoNormal" dir="rtl"><span dir="ltr"><!--stripped--><font face="times new roman, times, serif"> </font><!--stripped--></span></p> <p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><font face="times new roman, times, serif"><b><span style="font-size: 10pt color: blue">BACKGROUND AND OBJECTIVE:</span></b><span style="font-size: 10pt"> </span><span style="font-size: 10pt">Extended-spectrum beta-lactamase (ESBL) enzymes hydrolyze cephalosporins and penicillins. This study aimed to determine the frequency of <span style="background: white">Escherichia coli</span> strains producing SHV, TEM and CTX-M &beta-lactamase genes and their association by inducing antibiotic resistance</span><span style="font-size: 10pt">.<!--stripped--><!--stripped--></span></font></p> <p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><font face="times new roman, times, serif"><b><span style="font-size: 10pt color: blue">METHODS:</span></b><span style="font-size: 10pt"> </span><span style="font-size: 10pt">In this cross-sectional study, 55 E. coli strains were isolated from urinary samples </span><span style="font-size: 10pt">and cultured on</span><span style="font-size: 10pt"> <span style="background: white">eosin methylene blue </span>(EMB) agar and CHROMagar. After biochemical examinations, antibiotic susceptibility test was performed using the disk-diffusion method according to the guidelines of the Clinical & Laboratory Standards Institute (CLSI). In addition, the presence of </span><span style="font-size: 10pt">blaCTX-M, blaTEM and blaSHV</span><span style="font-size: 10pt"> genes was evaluated using specific multiplex polymerase chain reaction (PCR) primers</span><span style="font-size: 10pt">.<!--stripped--><!--stripped--></span></font></p> <p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><font face="times new roman, times, serif"><b><span style="font-size: 10pt color: blue">FINDINGS:</span></b><span style="font-size: 10pt"> </span><span style="font-size: 10pt">In this study, the highest antibiotic resistance was observed against penicillin and erythromycin (96% and 94.5%, respectively), while the highest susceptibility was reported for ciprofloxacin and imipenem (67.2%). Out of 55 samples, 26(47.27%) had the TEM gene, and CTX-M gene was detected in 41 (74.54%) samples. Moreover, TEM and CTX-M genes were simultaneously detected in 32.72% of the samples, while in six samples (10.9%), neither of these genes were present. The SHV gene was not detected in any of the samples</span><span style="font-size: 10pt">.<!--stripped--><!--stripped--></span></font></p> <p class="MsoNormal" style="line-height: 16pt direction: ltr unicode-bidi: embed"><font face="times new roman, times, serif"><b><span style="font-size: 10pt color: blue">CONCLUSION:</span></b><span style="font-size: 10pt"> </span><span style="font-size: 10pt">According to the results of this study, the production of ESBL was identified in 70% of the investigated E. coli isolates. Therefore, accurate and timely medical care, as well as the use of appropriate antibiotics, is required to prevent the outbreak of ESBL-producing </span><span style="font-size: 10pt">E. coli strains</span></font><span style="font-size: 10pt"><font face="times new roman, times, serif">.</font><!--stripped--><!--stripped--></span></p> اشریشیاکلی, بتالاکتاماز وسیع الطیف, دیسک دیفیوژن, PCR چندگانه‌ای. Escherichia coli, Extended-Spectrum Beta-Lactamase (ESBL), Disk-Diffusion, Multiplex PCR 19 26 http://jbums.org/browse.php?a_code=A-10-1851-1&slc_lang=fa&sid=1 Gh Miraalami قاسم میراعلمی Ghmiraalamy@gmail.com 100319475328460012442 100319475328460012442 Yes M Parviz مهدی پرویز 100319475328460012443 100319475328460012443 No S Khalajzadeh سعید خلج زاده 100319475328460012444 100319475328460012444 No