fa بررسی اثر ضد دردی و تعیین سمیت حاد عصاره متانولی گیاه رازیانه رومی (anisum.L Pimpinella) در موش صحرایی نر فارماکولوژی Pharmacology تجربی Experimental <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: 16pt direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">سابقه و هدف</span></b><b><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></b><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">رازیانه رومی</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;(</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">Pimpinella anisum.</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">)</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">، گیاهی دارویی است که به طور گسترده ای در طب سنتی ایران در درمان بیماری های گوارشی، التهابی و تشنجی</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مورد مصرف قرار می</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&shy;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">گیرد. این مطالعه به منظور بررسی اثر ضد&shy;دردی عصاره متانولی گیاه رازیانه رومی در موش صحرایی نر بالغ</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="FA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">انجام</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">شد</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span><span dir="ltr" style="font-size: 9pt font-family: Tahoma, sans-serif"><!--stripped--><!--stripped--></span></p> <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: 16pt direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مواد و روشها</span></b><b><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></b><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">در این مطالعه تجربی از 48 سر موش صحرایی نر در 8 گروه شامل گروه&shy;های: کنترل، گروه&shy;های تیمار شده با عصاره (50، 100 و200</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">mg/kg</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">)</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">، مورفین، آسپرین، نالوکسان به همراه عصاره و آسپرین به &shy;همراه عصاره استفاده شد</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">عصاره گیری گیاه به روش ماسراسیون انجام گرفته و</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">به</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">منظور ارزیابی اثرات ضد</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">دردی عصاره از آزمون&shy;های ریتینگ، تیل فلیک و فرمالین استفاده شد</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">همچنین کلیه تزریقات به صورت درون صفاقی انجام پذیرفت</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span><span lang="AR-SA" style="font-size: 9pt font-family: Tahoma, sans-serif"><!--stripped--><!--stripped--></span></p> <p class="MsoNormal" dir="rtl" style="margin-bottom: 0.0001pt line-height: 16pt direction: rtl unicode-bidi: embed"><b><span lang="AR-SA" style="font-size: 10pt font-family: '2 Mitra' color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">یافته&shy;ها</span></b><b><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></b><b><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span></b><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نتایج حاصل از مطالعه حاضر مشخص نمود که تزریق توام دوز 200</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;mg/kg</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">عصاره رازیانه رومی به همراه آسپرین توانست اثر ضد دردی معنی داری را با</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">p<0.05</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نسبت به آسپرین با کاهش زمان تاخیر به میزان 1 ثانیه در تست تیل فلیک نشان دهد. در تست ریتینگ دوز 200</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;mg/kg</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">عصاره توانست با کاهش تعداد انقباضات شکمی به عدد 10، اثر ضد دردی بیشتری را نسبت به به گروه آسپرین با</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">p<0.01</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نشان دهد. همچنین سمیت حاد عصاره گیاه 2125</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span dir="ltr" style="font-size: 10pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">mg/kg</span><span lang="AR-SA" style="font-size: 10pt font-family: 'B Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">بود</span><span dir="ltr" style="font-size: 9pt font-family: Tahoma, sans-serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span><span lang="AR-SA" style="font-size: 9pt font-family: Tahoma, sans-serif"><!--stripped--><!--stripped--></span></p> <p class="MsoNormal" dir="rtl" style="line-height: 16pt direction: rtl"></p> <p class="MsoNormal" dir="rtl" style="text-align: right direction: rtl unicode-bidi: embed"><b><span lang="FA" style="font-size: 10pt line-height: 115% font-family: '2 Mitra' color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نتیجه</span></b><b><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: 'Times New Roman', serif color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span></b><b><span lang="FA" style="font-size: 10pt line-height: 115% font-family: '2 Mitra' color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">گیری</span></b><b><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: Tahoma, sans-serif color: blue background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">:</span></b><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="FA" style="font-size: 10pt line-height: 115% font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نتایج</span><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt line-height: 115% font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">این</span><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="FA" style="font-size: 10pt line-height: 115% font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">مطالعه</span><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">&nbsp;</span><span lang="AR-SA" style="font-size: 10pt line-height: 115% font-family: '2 Mitra' background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">نشان داد که احتمالا عصاره متانولی برگ گیاه رازیانه رومی دارای اثر ضددردی (در هر دو فاز حاد و مزمن) می باشد</span><span dir="ltr" style="font-size: 10pt line-height: 115% font-family: 'Times New Roman', serif background-image: initial background-attachment: initial background-size: initial background-origin: initial background-clip: initial background-position: initial background-repeat: initial">.</span><span dir="ltr"><!--stripped--><!--stripped--></span></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p style="margin: 0cm 0cm 0pt text-align: justify line-height: 16pt unicode-bidi: embed direction: ltr -ms-text-justify: kashida text-kashida: 0% mso-line-height-rule: exactly"><font face="Times New Roman"><b><span style="color: blue font-size: 10pt">BACKGROUND AND OBJECTIVE:</span></b><span style="font-size: 10pt">&nbsp;</span><font size="2"><span style="mso-bidi-font-weight: bold">Pimpinella anisum is a medicinal herb widely used in the traditional Iranian medicine to cure digestive, inflammatory, and spastic diseases. This study aimed to evaluate the analgesic effects of methanol extract of Pimpinella anisum.L in mature male Wistar rats.</span></font></font></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p style="margin: 0cm 0cm 0pt text-align: justify line-height: 16pt unicode-bidi: embed direction: ltr mso-line-height-rule: exactly"><font face="Times New Roman"><b><span style="color: blue font-size: 10pt">METHODS:</span></b><span new="" style="color: red font-size: 10pt mso-bidi-font-family: " times="">&nbsp;</span><span style="font-size: 10pt mso-bidi-font-weight: bold">In this study, 48 male Wistar rats were divided into 8 groups of control, treated with the Pimpinella anisum.L extract (50, 100, 200 mg/kg), treated with morphine, aspirin, extract with naloxone and extract with aspirin. Extraction was performed via maceration, rating and tail flick tests. In addition, formalin tests were conducted to evaluate the analgesic effects of the extract, and all the injections were performed intraperitoneally</span><span new="" style="font-size: 10pt mso-bidi-font-family: " times="">.</span></font></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p style="margin: 0cm 0cm 0pt text-align: justify line-height: 16pt unicode-bidi: embed direction: ltr mso-line-height-rule: exactly"><font face="Times New Roman"><b><span style="color: blue font-size: 10pt">FINDINGS:</span></b><span new="" style="color: red font-size: 10pt mso-bidi-font-family: " times="">&nbsp;</span><span style="font-size: 10pt mso-bidi-font-weight: bold">Co-administration of 200 mg/kg of Pimpinella anisum.L extract combined with aspirin was observed to produce significant analgesic effects (p<0.05) compared to aspirin alone, with a one-second reduction of time delay in the tail flick test. In the rating test, 200 mg/kg of the extract resulted in more significant analgesic effects compared to aspirin alone (p<0.01), reducing the number of abdominal contractions to 10. The acute toxicity of the extract was determined as 2125 mg/kg.</span><span new="" style="color: red font-size: 10pt mso-bidi-font-family: " times="">&nbsp;</span></font></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> <p style="margin: 0cm 0cm 0pt text-align: justify line-height: 16pt unicode-bidi: embed direction: ltr -ms-text-justify: kashida text-kashida: 0% mso-line-height-rule: exactly"><font face="Times New Roman"><b><span style="color: blue font-size: 10pt">CONCLUSION:</span></b><span style="font-size: 10pt">&nbsp;</span><font size="2"><span style="mso-bidi-font-weight: bold">According to the results of this study, the methanol extract of Pimpinella anisum.L could exert analgesic effects in both the acute and chronic phases of pain</span></font><span new="" style="font-size: 10pt mso-bidi-font-family: " times="">.</span></font></p> <p><font face="Times New Roman" size="3">&nbsp;</font></p> واژه های کلیدی: ضد درد, عصاره متانولی, گیاه رازیانه رومی, فرمالین Analgesic, Methanol Extract, Pimpinella anisum.L, Pain 59 65 http://jbums.org/browse.php?a_code=A-10-1317-2&slc_lang=fa&sid=1 M Asgari nematian مهتاب عسگری نعمتیان 100319475328460013932 100319475328460013932 No S Mohammadi سعید محمدی sdmd3000@gmail.com 100319475328460013933 100319475328460013933 Yes mohammadi12214 100319475328460013934 100319475328460013934 No