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:: Volume 20, Issue 10 (10-2018) ::
J Babol Univ Med Sci. 2018; Volume 20 Back to browse issues page
Inhibitory Effect of Carvacrol on the Expression of Candida albicans Hyphae-Specific Gene (HWP1)
A Khodavandi , F Alizadeh * , Sh Zaboli zadeh
Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran. , mnalizadeh@yahoo.com
Abstract:   (3602 Views)
BACKGROUND AND OBJECTIVE: Candida albicans infection is a problem of growing clinical importance, particularly in immunocompromised populations. This study aimed to investigate the inhibitory effect of carvacrol on the hyphae formation and expression of C. albicans HWP1.
METHODS: This cross-sectional study was done over a 6-month period in 2016-2017. Vaginal, mouth and  skin surface swabs were obtained from immunocompromised patients. Colonizing clinical isolates of C. albicans were identified and drug susceptible isolates detected using WHONET software. The susceptibility test for carvacrol (range 6.25–300 μg/ml) were carried out with a broth microdilution according to the CLSI guidelines against drug susceptible C. albicans. The time kill assay of carvacrol (range 2 × MIC to ¼ × MIC) was determined. Hyphae inhibition was evaluated by light microscopy. We determined the expression levels of HWP1 implicated in hyphae formation of Calbicans ATCC 14053 cells by quantitative RT-PCR.
FINDINGS: Ten colonizing clinical isolates of drug susceptible C. albicans were identified. Carvacrol was inhibited the growth of all drugs susceptible isolates of C. albicans (MIC range, 25-200 µg/ml). Time kill curve assay demonstrated that carvacrol could significantly inhibit the growth of C. albicans (p≤0.05). Carvacrol efficiently prevented hyphae formation in drug susceptible C. albicans. The expression levels of HWP1 gene were down-regulated by 1.82- and 1.62-fold at concentrations of 2 × MIC and 1×MIC of carvacrol, respectively.
CONCLUSION: These results suggest that carvacrol could provide an improved and safe clinical approach in treating Candida infections by prevention of hyphae formation.          
Keywords: Candida albicans, Gene Expression, Hyphae
Full-Text [PDF 557 kb]   (1175 Downloads)    
Type of Study: Research | Subject: Biochemical
Received: 2018/03/25 | Accepted: 2018/10/23 | Published: 2018/11/10
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Khodavandi A, Alizadeh F, Zaboli zadeh S. Inhibitory Effect of Carvacrol on the Expression of Candida albicans Hyphae-Specific Gene (HWP1). J Babol Univ Med Sci 2018; 20 (10) :63-70
URL: http://jbums.org/article-1-7727-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 10 (10-2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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