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Comparision of Aqueous and Hydroalcoholic Extracts of Foeniculum Vulgare and Carum Copticum with Gentamicin on Escherichia Coli Strains: in Vitro Study
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F Abedi   , R Rajabnia , H Sorkhi , Z Memariani , H Shirafkan , SA Mozaffarpur * |
| 5. Traditional Medicine and History of Medical Sciences Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, I.R.Iran , seyyedali1357@gmail.com |
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Abstract: (4076 Views) |
BACKGROUND AND OBJECTIVE: Due to bacterial resistance to antibiotics, new antibacterial agents is essential. In Persian medicine Fennel (Fuenoculum vulgare Mill.) and Ajwain (Carum copticum (L.) Benth. & Hook.f.) are recommended for the treatment of some infections. In this research, bacteriostatic and bactericidal effects of aqueous and hydroalcoholic extracts of fennel and Ajwain on E. coli were investigated.
METHODS: In an in-vitro study 30 clinical isolates of urine culture of children with urinary tract infection from Amirkola Pediatric Hospital in Babol and a standard sample were used. Antibacterial effects of 4 grouos including aqueous and hydroalcoholic extracts of fennel and Ajwain by measuring the diameter of the inhibition zone using disc diffusion (concentrations 16, 32, 64, 128, 256 and 512 mg/disc) and determination of Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentration (MBC) with Microdilution method was compared with Gentamicin (30mg/disc) as a positive control
FINDINGS: There was no significant difference in inhibition zone with Gentamicin at concentrations of 64, 128, 256, and 512 mg/disc in standard and clinical samples. At concentrations of 16 and 32, Gentamicin was significantly better. The extract of 512 mg/disc (12.93±2.66) of hydroalcoholic extract of Carum copticum was significantly better than 256 mg/disc (9.53±1) (p=0.002). The MIC and MBC for standard samples were 4 and 8, respectively, and for clinical samples 3.83±2.36 and 5.8 mg / ml, respectively. Other extracts were not able to inhibit the growth of Escherichia coli.
CONCLUSION: The results showed that the Hydroalcoholic extract of Carum copticum has bacteriostatic and bactericidal effects on standard and clinical isolates of Escherichia coli. |
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| Keywords: Anti-Bacterial Agents, Ajwain, Fennel, Persian Medicine |
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Full-Text [PDF 203 kb]
(1813 Downloads)
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Type of Study: Case-Control |
Subject:
Virology
Received: 2017/11/20 | Accepted: 2018/03/4 | Published: 2018/03/4
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