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This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 
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:: Volume 20, Issue 6 (6-2018) ::
J Babol Univ Med Sci. 2018; Volume 20 Back to browse issues page
Effect of Isolated Tricin from Arial Part of Allium atrovoilaceum Boiss. on Proliferation and Apoptosis in PC3 Cell Line
Z Lorigooini , S Ghasemi * , N Asgarian Dehkordi , M Motamedi
2. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, I.R.Iran , sorayya.ghasemi@gmail.com
Abstract:   (4327 Views)
BACKGROUND AND OBJECTIVE: Tricin as a flavone compound, in many food sources, has anti-inflammatory and anti-proliferative effects in several cancer cell lines. Considering the importance of using natural anti-cancer drugs in treatment-resistant cancers such as prostate cancer, the aim of this study was to investigate the effect of tricin on cell growth and proliferation and induction of apoptosis in human PC3 prostate cancer cell line.
METHODS: In this experimental study, the PC3 cell line was prepared from the Pasteur Institute of Iran and cultivated. Extraction and purification of Tricin were performed using column chromatography and re-crystallization of Alliuum atrovoilaceum extract. The cytotoxic effect of Tricin in concentrations of 60,80,100,120, and 140 μM was evaluated by MTT method. Apoptotic effect was evaluated in the treated cell group with the IC50 concentration of Tricin and untreated cells (control group), using an Annexin-V kit and flow cytometry.
FINDINGS: The viability of cells at different tricin concentrations were 85.66±1.52, 76±3.60, 66.33±4.16, 44±3.60, and 36.66±3.21, respectively (p<0.01). The IC50 concentration of tricin was 117.5±4.4 μM for PC3 cell line. The apoptosis rate in PC3 cells after 48 hours of treatment with IC50 concentration of tricin was 24.3±0.58%, which was not significant in comparison with control cells (23.3±0.58%). 
CONCLUSION: The results of this study showed that Tricin resulted in cell death in the PC3 cell line, but the cell death mechanism was not apoptosis.
Keywords: Prostate Cancer, Tricin, Apoptosis
Full-Text [PDF 350 kb]   (998 Downloads)    
Type of Study: Experimental | Subject: Genetics, Cell and Molecular Biology
Received: 2017/10/4 | Accepted: 2018/04/17 | Published: 2018/05/13
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Lorigooini Z, Ghasemi S, Asgarian Dehkordi N, Motamedi M. Effect of Isolated Tricin from Arial Part of Allium atrovoilaceum Boiss. on Proliferation and Apoptosis in PC3 Cell Line. J Babol Univ Med Sci 2018; 20 (6) :36-40
URL: http://jbums.org/article-1-7177-en.html


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Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 20, Issue 6 (6-2018) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

The Journal of Babol University of Medical Sciences is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
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