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:: Volume 18, Issue 8 (8-2016) ::
JBUMS 2016, 18(8): 41-47 Back to browse issues page
A Study of Mechanism and Rate of PC12 Cancer Cell Destruction Induced by Lysine-Coated Gold Nanoparticle
K Shahzamani , H Zare marzouni , F Tarkhan , HE Lashgarian
Hepatitis Research Center, Lorestan University of Medical Science , Khorramabad, I.R. Iran , hamedesmaiili@gmail.com
Abstract:   (2929 Views)

BACKGROUND AND OBJECTIVE: Using gold nanoparticles for cancer treatment has attracted the attention of many researchers in recent years. Entry of these nanoparticles into cells is one the problems that influences the effectiveness of the treatment. The purpose of this study is to identify the destructive effects of amino acid lysine-coated gold nanoparticles on PC12 cancer cells.

METHODS: In this empirical study, gold nanoparticles were prepared using low temperature hydrothermal technique. After obtaining PC12 cells from Pasteur Institute of Iran and culturing them, we divided them into 7 treatment groups with concentrations of 0.5, 2.5 and 5 μM gold nanoparticles, with and without lysine coating; plus one control group. The cytotoxic effect of nanoparticles was assessed using MTT assay and LDH release from cells. In addition, cell morphology and amount of ROS (reactive oxygen species) within cells were examined.

FINDINGS: According to the results of this study, a 40% increase in cell death occurred as a result of exposure to 25 μM amino acid lysine-coated gold nanoparticles, which was significantly higher than the group treated by uncoated gold nanoparticles (18% cell death) (p≤0.001). In addition, incubating cells with coated and uncoated nanoparticles increased the amount of ROS and mechanism of cell death through apoptosis.

CONCLUSION: Results of the study revealed that lysine-coated gold nanoparticles have promising effects on destruction of PC12 cancer cells and they direct cell death towards apoptosis.

Keywords: Cancer, Nanoparticle, Lysine, Apoptosis
Full-Text [PDF 355 kb]   (966 Downloads)    
Type of Study: Experimental | Subject: Nano
Received: 2016/01/3 | Accepted: 2016/08/10 | Published: 2016/08/30
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Shahzamani K, Zare marzouni H, Tarkhan F, Lashgarian H. A Study of Mechanism and Rate of PC12 Cancer Cell Destruction Induced by Lysine-Coated Gold Nanoparticle. JBUMS. 2016; 18 (8) :41-47
URL: http://jbums.org/article-1-5885-en.html

Volume 18, Issue 8 (8-2016) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی بابل Journal of Babol University of Medical Sciences

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