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A Study of the Bactericidal Effect of Copper Oxide Nanoparticles on Shigella Sonnei and Salmonella Typhimurium
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S Babaei   , F Bajelani , O Mansourizaveleh , A Abbasi , F Oubari * |
| Kermanshah University of Medical Sciences,Kermanshah ,Iran. , Foubari@mbrc.ac.ir |
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Abstract: (5451 Views) |
BACKGROUND AND OBJECTIVE: Microbial resistance is one of the most important challenges in dealing with infectious diseases. Therefore, finding or synthesizing new antimicrobial agents is very important. Copper oxide (CuO) is considered for its antibacterial effect against microbial resistance. This study was conducted to investigate the antibacterial effects of copper oxide nanoparticles on shigella sonnei and salmonella typhimurium bacteria, which have new strains associated with microbial resistance.
METHODS: In this applied fundamental research, copper oxide nanoparticles were synthesized from copper sulfate in sizes of 33 and 56 nm, using a chemical reduction method. Then, the antibacterial effects of copper oxide nanoparticles on the standard strain of shigella sonnei (ATCC–9290) and salmonella typhimurium (PTCC–1609) were investigated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and bacterial death kinetics.
FINDINGS: The MIC obtained in shigella sonnei and salmonella typhimurium treatment with a 33 nm nanoparticle were 2500mg/ml and 5000 mg/ml, respectively, and the value for 56 nm nanoparticle for both bacteria was 5,000mg/ml. The obtained MBC in the treatment of shigella sonnei and salmonella typhimurium using 33 nm nanoparticle was 5000≤IU/ml and 10,000≤IU/ml, respectively, and the same for 56 nm nanoparticle for both bacteria was equal to 10,000≤IU/ml.
CONCLUSION: The research proves that copper oxide nanoparticles have a bactericidal effect on shigella sonnei and salmonella typhimurium, and that the bactericidal effect of smaller nanoparticles is greater than that of bigger nanoparticles, while the antibacterial effects on shigella sonnei was more significant.
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| Keywords: Copper Oxide Nanoparticle, Shigella Sonnei, Salmonella Typhimurium, MIC, MBC, Bacterial Death Kinetics |
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Full-Text [PDF 475 kb]
(1624 Downloads)
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Type of Study: Research |
Subject:
Microbiology
Received: 2017/05/13 | Accepted: 2017/10/29 | Published: 2017/11/12
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